TABLE 5.
Primers used to construct the double-mutant ST0452 proteins
| Primer name | Sequencea |
|---|---|
| dT80AF | TAAAAGGGGCGGGTGCGGCCATATTGTCTG |
| dT80AR | GCCGCACCCGCCCCTTTTATATCATCTTTC |
| dY97AF | CACTTATAATTGCGGGAGATTTATTCTTTTC |
| dY97AR | TAAATCTCCCGCAATTATAAGTGCTTCATCG |
| dY97FF | CTTATAATTTTTGGAGATTTATTCTTTTCAAAC |
| dY97FR | AATCTCCAAAAATTATAAGTGCTTCATCG |
| dK147AF | ATTATAGAAGCGCCAGAGATACCTCCATC |
| dK147AR | GTATCTCTGGCGCTTCTATAATTTTAGATAAG |
a
Underlining indicates the overlap regions introduced into each primer. Boldface indicates the substituted nucleotides.