TABLE 6.
List of primer sets and template plasmids used to construct the double-mutant ST0452 genes with names of the product plasmids and proteins
| Forward primer | Reverse primer | Template plasmid | Product plasmid | Protein name |
|---|---|---|---|---|
| dT80AF | dT80AR | pST0452(G9A)H | pST0452(G9A)(T80A)H | G9A/T80A |
| dY97AF | dY97AR | pST0452(G9A)H | pST0452(G9A)(Y97A)H | G9A/Y97A |
| dY97FF | dY97FR | pST0452(G9A)H | pST0452(G9A)(Y97F)H | G9A/Y97F |
| dK147AF | dK147AR | pST0452(G9A)H | pST0452(G9A)(K147A)H | G9A/K147A |
| dY97AF | dY97AR | pST0452(T80A)H | pST0452(T80A)(Y97A)H | T80A/Y97A |
| dY97FF | dY97FR | pST0452(T80A)H | pST0452(T80A)(Y97F)H | T80A/Y97F |
| dK147AF | dK147AR | pST0452(T80A)H | pST0452(T80A)(K147A)H | T80A/K147A |
| dK147AF | dK147AR | pST0452(Y97A)H | pST0452(Y97A)(K147A)H | Y97A/K147A |
| dK147AF | dK147AR | pST0452(Y97F)H | pST0452(Y97F)(K147A)H | Y97F/K147A |