TABLE 8.
List of primer sets, template plasmids used for construction of substitution mutant ST0452 genes, plasmids used as templates, and names of the product plasmids and proteins
| Forward primer | Reverse primer | Template plasmid | Plasmid name | Protein name |
|---|---|---|---|---|
| dY97CF | dY97CR | pST0452H | pST0452(Y97C)H | Y97C |
| dY97DF | dY97DR | pST0452H | pST0452(Y97D)H | Y97D |
| dY97EF | dY97ER | pST0452H | pST0452(Y97E)H | Y97E |
| dY97GF | dY97GR | pST0452H | pST0452(Y97G)H | Y97G |
| dY97HF | dY97HR | pST0452H | pST0452(Y97H)H | Y97H |
| dY97IF | dY97IR | pST0452H | pST0452(Y97I)H | Y97I |
| dY97KF | dY97KR | pST0452H | pST0452(Y97K)H | Y97K |
| dY97LF | dY97LR | pST0452H | pST0452(Y97L)H | Y97L |
| dY97MF | dY97MR | pST0452H | pST0452(Y97M)H | Y97M |
| dY97NF | dY97NR | pST0452H | pST0452(Y97N)H | Y97N |
| dY97PF | dY97PR | pST0452H | pST0452(Y97P)H | Y97P |
| dY97QF | dY97QR | pST0452H | pST0452(Y97Q)H | Y97Q |
| dY97RF | dY97RR | pST0452H | pST0452(Y97R)H | Y97R |
| dY97SF | dY97SR | pST0452H | pST0452(Y97S)H | Y97S |
| dY97TF | dY97TR | pST0452H | pST0452(Y97T)H | Y97T |
| dY97VF | dY97VR | pST0452H | pST0452(Y97V)H | Y97V |
| dY97WF | dY97WR | pST0452H | pST0452(Y97W)H | Y97W |