TABLE 9.
Primers used to construct the expression vector for wild-type and mutant EcGlmU proteins
| Primer name | Sequencea |
|---|---|
| dEcGlmUF | ACTGGcatatgTTGAATAATGCTATGAGCG |
| dEcGlmUR | TCAActcgagCTTTTTCTTTACCGGACGAC |
| dEcGlmUY103AF | TGCTCGCGGGCGACGTGCCGCTGATCTCTG |
| dEcGlmUY103AR | GTCGCCCGCGAGCATTAAAATGTCTTCATC |
| dEcGlmUY103NF | TGCTCAATGGCGACGTGCCGCTGATCTCTG |
| dEcGlmUY103NR | GTCGCCATTGAGCATTAAAATGTCTTCATC |
| dEcGlmUY103VF | TGCTCGTGGGCGACGTGCCGCTGATCTCTG |
| dEcGlmUY103VR | GTCGCCCACGAGCATTAAAATGTCTTCATC |
a
Lowercase letters indicate the NdeI site, and italicized lowercase letters indicate the XhoI site. Underlining indicates the overlap regions introduced into each primer. Boldface indicates substitutions.