FIG 3.

Anti-C VHH PVSP17B can bind to purified 80S and 135S poliovirions but not to 160S native virions. Poliovirions, in various states of expansion, were incubated at 25°C, first with the VHH in solution and then with Ni-NTA-agarose beads that bind to the His₆ tail of the VHH. After washing, poliovirus and VHH proteins were eluted with imidazole and visualized as characteristic bands on SDS-PAGE gels. Lane 1, EZ ladder size standards (with masses listed in kilodaltons at the left); lane 2, 160S PV particles plus VHH; lane 3, 135S PV particles plus VHH; lane 4, 80S PV particles plus VHH; lane 5, VHH (without PV); lane 6, 135S PV particles (without VHH); lane 7, purified 160S PV particles (control); lane 8, purified VHH (control). Native poliovirus (lane 7) shows characteristic bands for VP1, VP2, VP3, and VP4 (top to bottom).