FIG 5.

NS1, REH, and cis elements of (pA)d are required for polyadenylation at (pA)d2. (A) Diagram of (pA)d reporter plasmids. (pA)d and downstream sequence were inserted into pEGFP-C1 to replace the SV40 polyadenylation signal. (B) Northern blot analysis. Total RNA was isolated from transfected HEK293T cells, and Northern blotting was performed as described in the legend to Fig. 2D. (C) Western blot. HEK293T cells were transfected with an HA-tagged NS1 expression plasmid and analyzed using Western blotting 48 h posttransfection.