FIG 7.

Digoxin and digitoxin block adenovirus genome replication. A549 cells were infected with HAdV-C5 and treated with 100 nM digoxin (A) or 100 nM digitoxin (B) immediately after the adsorption period. Cells were collected at 0, 10, 16, and 20 or 22 h p.i., total DNA was extracted, and the level of adenovirus DNA was determined by qPCR. The relative amount of viral DNA at each time point is plotted as the ratio of viral DNA to cellular TBP DNA (E3/TBP). The results represent three independent experiments for digoxin (A) and two independent experiments for digitoxin (B), with each sample analyzed in duplicate. Error bars show the standard error of the mean.