FIG 3.

Expression of cloned wild-type chNHE1 and its hybrid variants with ECL1 of New World quails, but not duck, confers susceptibility to ALV-J on QT6 cells. QT6 cells were transfected with NHE1 expression constructs and were infected with RCASBP(J)GFP on the next day. The percentage of GFP-positive cells was analyzed by flow cytometry 2 days postinfection. The RCASBP(J)GFP susceptibility conferred on QT6 cells by NHE1 hybrid variants containing ECL1 derived from New World quail NHE1 may be compared to that conferred by chicken NHE1 or hybrid NHE1 containing ECL1 from duck by observing the percentages of efficiently transfected tdTomato-positive cells that were GFP positive. Results were calculated as the averages for three parallel wells ± standard deviations.