TABLE 2.
Primers for PCR, site-directed mutagenesis, and real-time PCR in this study
| Primer name and function | Sequencea |
|---|---|
| Primers for PCR | |
| pCD163-SRCR5, forward-1 | GATCTCCCAGGCTGGTTGGAGG |
| pCD163-SRCR5, forward-2 | TCCCAGGCTGGTTGGAGG |
| pCD163-SRCR5, reverse | CGACGCGTTGAGCAGACTACGCCGACG |
| Site-directed mutagenesis | |
| pCD163-R478A, forward | GCTCAGCCCACAGGAAACCCgccCTGGTTGGAGGGGACATTC |
| pCD163-R478A, reverse | GAATGTCCCCTCCAACCAGggcGGGTTTCCTGTGGGCTGAGC |
| pCD163-D483A, forward | CCCAGGCTGGTTGGAGGGgccATTCCCTGCTCTGGTCGTG |
| pCD163-D483A, reverse | CCCAGGCTGGTTGGAGGggcCATTCCCTGCTCTGGTCGTG |
| pCD163-D496A, forward | GTTGAAGTACAACATGGAgccACGTGGGGCACCGTCTGTG |
| pCD163-D496A, reverse | CACAGACGGTGCCCCACGTggcTCCATGTTGTACTTCAAC |
| pCD163-R561A, forward | CACTCTGCCCAGTAGCACCCgccCCTGACGGGACATGTAGC |
| pCD163-R561A, reverse | GCTACATGTCCCGTCAGGggcGGGTGCTACTGGGCAGAGTG |
| pCD163-D563A, forward | CCCAGTAGCACCCCGCCCTgccGGGACATGTAGCCACAGCAG |
| pCD163-D563A, reverse | CTGCTGTGGCTACATGTCCCggcAGGGCGGGGTGCTACTGGG |
| pCD163-R570A, forward | CGGGACATGTAGCCACAGCgccGACGTCGGCGTAGTCTGCTC |
| pCD163-R570A, reverse | GAGCAGACTACGCCGACGTCggcGCTGTGGCTACATGTCCCG |
| Quantitative real-time PCR | |
| PRRSV-ORF7, forward | AAACCAGTCCAGAGGCAAGG |
| PRRSV-ORF7, reverse | GCAAACTAAACTCCACAGTGTAA |
| GAPDH, forward | CCTTCCGTGTCCCTACTGCCAAC |
| GAPDH, reverse | GACGCCTGCTTCACCACCTTCT |
a
The boldface letters indicate restriction sites, and the lowercase letters indicate mutated sites.