TABLE 1.

IC₅₀s of soluble CD4 and CD4mc for Envs from different HIV-1 strains or AMLV

^a The cytotoxicity of JP-III-48 and BNM-III-170 for Cf2Th-CD4/CCR5 cells was tested with the CellTiter-Glo luminescent cell viability assay. No cytotoxicity was detected at JP-III-48 and BNM-III-170 concentrations up to 300 μM.

^b In a pilot experiment, we varied the amount of the HIV-1_JR-FL Env-expressing plasmid transfected into the 293T cells producing recombinant luciferase-expressing HIV-1 up to 100-fold. No significant effect of the amount of Env on the IC₅₀ of BNM-III-170 was observed (data not shown). We determined that the IC₅₀ for BNM-III-170 inhibition of a single-round infection of recombinant HIV-1 with the SF162-P3 Env ranged from 14.7 to 19.8 μM. These IC₅₀s are relevant to the inhibition of replication-competent immunodeficiency viruses. For example, we found that the replication of a simian-human immunodeficiency virus with the SF162-P3 Env in rhesus macaque peripheral blood mononuclear cells was completely inhibited at 50 to 100 μM concentrations of BNM-III-170 (data not shown).