FIG 10.

Sequence alignment of wild-type AAPs and the mutated AAPs encoded by the AAP ORFs within the cmVP3 ORFs. Amino acid sequences encoded by the mutated AAP ORFs in the pCMV₁-AAVxcmVP3 plasmids (where x is 2, 4, 5, and 11) (knockout [KO]) are aligned with the wild-type (WT) AAPs, showing extensive amino acid alterations with new stop codons in the mutated AAP ORFs within the cmVP3 ORFs. Black and red letters indicate amino acids with and without changes, respectively. Underlining indicates stop codons. Annotations and the indicated amino acid positions were described previously by Naumer et al. (14). HR, hydrophobic region; CC, conserved core; PR, proline rich; TSR, T/S rich; BR, basic region.