TABLE 1.
Theoretically and experimentally determined molecular masses of FLAG-tagged AAPs
AAP | Theoretical molecular mass (kDa) | Mean experimentally determined molecular mass (kDa) ± SDa | % difference between theoretical and experimentally determined molecular massesb |
---|---|---|---|
1 | 22 | 27.3 ± 1.7 | 24 |
2 | 24 | 27.3 ± 1.6 | 14 |
3B | 23 | 28.5 ± 1.2 | 24 |
4 | 19 | 31.0 ± 1.1 | 63 |
5 | 22 | 33.2 ± 0.9 | 51 |
6 | 22 | 27.3 ± 1.3 | 24 |
7 | 22 | 27.8 ± 1.7 | 26 |
8 | 22 | 30.4 ± 1.5 | 38 |
9 | 22 | 33.7 ± 0.7 | 53 |
10 | 22 | 34.4 ± 0.7 | 56 |
11 | 20 | 29.7 ± 0.6 | 48 |
12 | 20 | 28.6 ± 0.7 | 43 |
The molecular mass of each FLAG-tagged AAP was determined by Western blot analysis in four biological replicate experiments.
Percent increase of the molecular mass as determined by Western blot analysis compared to the theoretical molecular mass.