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. 2017 Jan 18;91(3):e01980-16. doi: 10.1128/JVI.01980-16

TABLE 1.

Theoretically and experimentally determined molecular masses of FLAG-tagged AAPs

AAP Theoretical molecular mass (kDa) Mean experimentally determined molecular mass (kDa) ± SDa % difference between theoretical and experimentally determined molecular massesb
1 22 27.3 ± 1.7 24
2 24 27.3 ± 1.6 14
3B 23 28.5 ± 1.2 24
4 19 31.0 ± 1.1 63
5 22 33.2 ± 0.9 51
6 22 27.3 ± 1.3 24
7 22 27.8 ± 1.7 26
8 22 30.4 ± 1.5 38
9 22 33.7 ± 0.7 53
10 22 34.4 ± 0.7 56
11 20 29.7 ± 0.6 48
12 20 28.6 ± 0.7 43
a

The molecular mass of each FLAG-tagged AAP was determined by Western blot analysis in four biological replicate experiments.

b

Percent increase of the molecular mass as determined by Western blot analysis compared to the theoretical molecular mass.