FIG 2.

Infection of MARC-145 cells with PRRSV type 1 and type 2 strains reduces the STAT3 protein level but has a minimal effect on STAT3 transcripts. (A) Reduction of STAT3 protein level but no effect on STAT1 protein level in MARC-145 cells infected with PRRSV type 2 strains. The cells were infected with PRRSV type 2 strains VR-2385 (VR), Ingelvac PRRS MLV, and VR-2332 (VR1). At 48 hpi, the cells were harvested for WB with antibody against STAT3, STAT1, and tubulin. The relative levels of STAT3 are shown below the images after normalization with tubulin. (B) Reduction of STAT3 protein level by PRRSV type 1 strain Lelystad. (C) STAT3 mRNA levels in PRRSV-infected cells. The relative STAT3 mRNA levels are shown compared to the mock-infected cells. Error bars represent standard errors of the means of three repeated experiments. LV, Lelystad.