FIG 3.

PRRSV reduces STAT3 protein level in a dose- and time-dependent manner. (A) Dose-dependent reduction of STAT3 by PRRSV strain VR-2385. MARC-145 cells were inoculated with incremental MOIs of VR-2385 and harvested for WB with antibodies against STAT3, STAT1, and tubulin at 48 hpi. The relative levels of STAT3 are shown below the images after normalization with tubulin. (B) PRRSV RNA levels in infected cells. The cells were harvested for RNA isolation and RT-qPCR at 24 hpi. Error bars represent the standard errors of the results of repeated experiments. Significant differences in RNA level from an MOI of 0.01 are denoted by asterisks (**, P < 0.01; ***, P < 0.001). (C) Temporal kinetics of STAT3 levels in PRRSV-infected cells. MARC-145 cells were infected with VR-2385 at an MOI of 1. At the different time points, the cells were harvested for WB. The relative levels of STAT3 protein are shown below the images after normalization with tubulin at each time point.