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Substrate identification for ELISA to detect pUL89-C activity. (A) pUC18 or pUC18 digested with DpnI was incubated with (+) or without (−) pUL89-C. (B) The band intensity on an agarose gel was analyzed by using ImageJ. A band intensity ratio (absence of pUL89/presence of pUL89) of >1.5 was considered a high cleavage efficiency. (C) Three different sizes of dsDNA fragments incubated with or without pUL89-C. (D) Schematic of the ELISA format for analysis of pUL89-C activity.
