FIG 4.

HPCA compound 10k is a pUL89-C and HCMV inhibitor. (A) Dose-response inhibition of compound 10k for pUL89-C nuclease activity evaluated by an ELISA, stopping the reaction at either 15 or 60 min. (B) Dose-response inhibition of pUL89-C nuclease activity by compound 10k with an agarose gel readout. (C) Schematic representation of the experimental setup. HFF cells were inoculated with HCMV ADCREGFP and treated with serial dilutions of compounds. After 144 h, cell supernatants were collected for a yield reduction assay, and the original inoculated cells were analyzed for GFP expression as a measure of virus replication. For the yield reduction assay, HFF cells were inoculated with the supernatants from the antiviral assay, and GFP expression was measured after 144 h. (D and E) Representative dose-response curves for compound 10k in an HCMV replication assay (D) and a yield reduction assay (E).