FIG 8.
Functional viral DNA cleavage assay. (A) Schematic representation of the experimental setup. The position of the cleavage DNA probe (genome positions 703 to 1524) is indicated by a gray rectangle. Isolated viral DNA was digested, size fractionated by gel electrophoresis, and analyzed by Southern blotting. Concatemeric viral DNA yields an ∼5.5-kb fragment, whereas terminase-cleaved DNA yields an ∼2-kb fragment. The drawing is not to scale. (B) HFF cells were inoculated with HCMV ADCREGFP at an MOI of 0.1 and treated with ∼10× EC50 of letermovir or compound 10k. Ctrl, control (DMSO treatment).