Fig. 4.

Analytical equilibrium sedimentation in C12E8 micelles with density matching using 18% D₂O. (A) Peptide TH1 sediments with an apparent molecular weight of a dimer. (B) TF1 has an apparent molecular weight that is a little more than a tetramer. (C) Peptide TH2 sediments as a monomer under these conditions. (D) On the other hand, the fluorinated interface in TF2 drives the formation of dimers. Data were fit to an idealized single species model; solid lines calculated for monomer or oligomer assemblies are indicated by labels. All experiments were run at pH 7.0, [peptide] = 10 μM, [C12E8] = 5 mM, 20 mM phosphate buffer, 18% D₂O, and 200 mM NaCl, at 25°C and 40,000 rpm.