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. 2017 Jan 19;7:40960. doi: 10.1038/srep40960

Figure 1. Human p53 interacts with RPB1 both under normal circumstances and upon ActD treatment.

Figure 1

(A,B) The transcriptionally active forms of RPB1 (S2P and S5P) were immunoprecipitated and the RPB1-p53 interaction was validated by detection of p53 and S15P p53 with immunoblot using specific antibodies. (C) Asterisks show the unspecific signal of the IgG heavy chain. The reversal immunoprecipitation was performed by a p53-specific antibody. The p53-S2P RPB1 and p53-S5P RPB1 interactions were detected by specific antibodies against S2P and S5P RPB1 while IgG controls were used as the negative controls. (D,E) Western blots were performed on the input protein samples to prove the equal protein quantity for each sample used for the S2P S5P RPB1 (D) and p53 (E) immunoprecipitations. GAPDH was used to show the equal loading of the samples.