Fig. 3.

CD4⁺CD25⁺ cells in SJL and B10.S mice are functional. (a) CD4⁺CD25⁺ and CD4⁺CD25^- fractions were enriched from naïve SJL and B10.S mice. The different populations, either alone or together, were stimulated with anti-CD3 Ab in the presence of APC, and proliferative responses were measured as cpm. (b) CD4⁺CD25⁺ and CD4⁺CD25^- populations as in a were stimulated with PLP 139-151 (150 μg/ml) in the presence of APC, and proliferative responses were measured. A representative experiment from three to five individual experiments is shown in each graph.