Fig. 4.
Depletion of CD25+ cells in vivo enhances PLP 139-151-specific T cell responses in B10.S mice. (a) Proliferation. CD3+ T cells from PLP 139-151-immunized B10.S mice that had been treated with anti-CD25 Ab or rat IgG control were restimulated with PLP 139-151, and proliferative responses were measured. (b) IAs tetramer staining. CD3+ T cells from PLP 139-151-immunized B10.S mice that were pretreated with anti-CD25 Ab or rat IgG were restimulated with PLP 139-151 for 4 days. The percentages of IAs tetramer (PLP 139-151, TMEV 70-86)-positive cells were determined in the viable (7-AAD-) CD4 subset. A representative experiment from five individual experiments involving two mice per group is shown.