Table 1.
Primer | Forward sequence | Reverse sequence | Size | Temp (C) | Species | Reference ID |
---|---|---|---|---|---|---|
18S | GTAACCCGTTGAACCCCATT | CCATCCAATCGGTAGTAGCG | 152 | 60 | Bovine, Human | NR_036642.1 |
COLI | CGGCTCCTGCTCCTCTTAG | CACACGTCTCGGTCATGGTA | 137 | 60 | Bovine, Human | NM 001034039.1; NM_000088.3 |
COL2 | GTGTCAGGGCCAGGATGTC | GCAGAGGACAGTCCCAGTGT | 127 | 60 | Bovine, Human | NM 001001135.2; NM_001844.4 |
ACAN | TGGGACTGAAGTTCTTGGAGA | GCGAGTTGTCATGGTCTGAA | 133 | 60 | Bovine, Human | NM 173981.2; NM_001135.3 |
CCND1 | CCTCTCCTATCACCGCCTGA | TTTGGGGTCCAAGTTCTGCT | 142 | 60 | Bovine | NM_001046273.2 |
KI67 | GAGACAGCCCAGGACACTTC | CCTGGTTCTCTGCACCATGT | 114 | 60 | Bovine | XM_002698582.2; XM_005225873.1 |
CCND1 | CACGGACTACAGGGGAGTTT | CTCTGCTGCTCGCTGCTA | 85 | 60 | Human | NM_053056.2 |
KI67 | ACAGTACCGCAGATGACTCAA | CGTCCAGCATGTTCTGAGGA | 76 | 60 | Human | NM_002417.4 |
CFL | CCCCTGAGTGTGCACCCCTTAA | CCCCCAGCTTCTCTGCAAGGGT | 150 | 60 | Bovine | NM_001015655.1 |