Table 2. Analysis of the markers of biomolecular pathway of damage induced by bilirubin.
| Hip | Cll | IC | SC | Ctx | |
|---|---|---|---|---|---|
| Ho1 | 3.97 ± 1.09c | 2.55 ± 0.95b | 3.39 ± 0.92c | 2.51 ± 0.67a | 1.65 ± 0.09 |
| Srnx1 | 14.90 ± 2.79c | 4.38 ± 3.15a | 17.42 ± 5.65a | 9.12 ± 4.83b | 1.44 ± 1.12 |
| Tnfα | 3.58 ± 2.17a | 2.06 ± 1.3 | 1.81 ± 0.94 | 2.25 ± 0.32c | 1.08 ± 0.61 |
| Il1β | 13.69 ± 7.17b | 8.83 ± 6.08a | 23.96 ± 10.58c | 5.38 ± 2.8c | 2.89 ± 1.51a |
| Il6 | 305.08 ± 120.22c | 9.64 ± 5.04c | 98.92 ± 38.35c | 122.08 ± 126.10a | 12.39 ± 7.61c |
| Cox2 | 53.64 ± 11.12c | 62.19 ± 29.95c | 56.08 ± 21.88c | 2.48 ± 2.06a | 3.53 ± 2.44a |
| Glu | 2.39 ± 0.31c | 1.20 ± 0.25 | 2.03 ± 0.59b | 1.23 ± 0.18 | 1.21 ± 0.18 |
The expression of selected genes, used as markers of biomolecular pathways of damage induced by bilirubin, was expressed as fold change of the control (DMSO-challenged OBCs). Similarly, quantification of glutamate release in the medium of Bf-challenged OBCs was expressed as the fold change of DMSO-exposed cultures. Ho1: heme oxygenase1; Srnx1: sulfiredoxin 1; Tnfα: tumor necrosis factor alfa; Il1β: interleukin 1β; Cox2: cyclo-oxygenase 2. Data are expressed as the mean ± SD of three-five repetitions. Statistical significance: ap < 0.05; bp < 0.01; cp < 0.001. Hip: hippocampus; Cll: cerebellum; IC: inferior colliculus; SC: superior colliculus; Ctx: cortex.