Table 4. Primer specifications.
| Gene | Accession number | Forward primer 5′-3′ | Reverse primer 3′-5′ | Amplicon length (bp) |
|---|---|---|---|---|
| Hprt | NM_012583.2 | AGACTGAAGAGCTACTGTAATGAC | GGCTGTACTGCTTGACCAAG | 163 |
| Gapdh | NM_017008.2 | CTCTCTGCTCCTCCCTGTTC | CACCGACCTTCACCATCTTG | 87 |
| Ho1 | NM_012580.2 | GGTGATGGCCTCCTTGTA | ATAGACTGGGTTCTGCTTGT | 76 |
| Srxn1 | NM_001047858.3 | AAGGCGGTGACTACTACT | TTGGCAGGAATGGTCTCT | 85 |
| Tnfα | NM_012675.2 | CAACTACGATGCTCAGAAACAC | AGACAGCCTGATCCACTCC | 172 |
| IL1β | NM_031512.2 | AACAAGATAGAAGTCAAGA | ATGGTGAAGTCAACTATG | 137 |
| IL6 | NM_012589.1 | GCCCACCAGGAACGAAAGTC | ATCCTCTGTGAAGTCTCCTCTCC | 161 |
| Cox2 | NM_017232.3 | CTTTCAATGTGCAAGACC | TACTGTAGGGTTAATGTCATC | 92 |
Primer sequences designed for the mRNA quantification. Hprt hypoxanthine guanine phosphoribosyltransferase, Gapdh glyceraldehyde 3-phosphate dehydrogenase, Hmox1 Heme oxygenase 1, Sxrn1 sulfiredoxin 1, Tnfα tumor necrosis factor alpha, Il1β interleukin 1 beta, Il6 interleukin 6, Cox2 cyclooxygenase 2.