16S rRNA gene-based phylogenetic tree of the family Peptostreptococcaceae. The sequences
from type strains (indicated with superscript T) were used and listed under their DSM
accession numbers, where available. GenBank accession numbers are listed in
parentheses. For Clostridioides
difficile 630, Clostridioides mangenotii TR,
Paraclostridium benzoelyticum
JC272T, and ‘Terrisporobacter othiniensis’,
16S rRNA gene sequences were taken from the respective genomic entries. Quotation
marks indicate the organisms whose names have not yet been validly published. The
organisms that this work proposes to be renamed are indicated in boldtype. The
sequences were aligned using muscle (Edgar,
2004) and the tree was inferred using the maximum-likelihood method based
on the Tamura–Nei model (Tamura & Nei,
1993) as implemented in mega6 (Tamura et al., 2013); for the initial neighbour-joining
tree, see Fig. S1. The tree was rooted using sequences from C.
butyricum and C.
botulinum, members of
Clostridium sensu stricto.