EBOV-positive samples for RNA-seq (n = 138) |
Poor quality: removed from analysis (n = 26) |
N/A |
N/A |
Good-quality samples (n = 112) |
Survived (n = 24) |
Transcriptomic analysis for differential gene expression and classifier generation |
Fatal (n= 88) |
EBOV-positive and malaria-negative (n = 37) |
N/A |
Fatal (n = 20) |
Flow analysis |
Survived (n = 17) |
Febrile and EBOV-negative (n = 10) |
N/A |
N/A |
Malaria-positive and EBOV-negative (n = 5) |
N/A |
N/A |
EBOV-positive sample with similar Ct values (n = 20) |
Good quality (n = 20) |
Survived (n = 10) |
Validation dataset for differential gene expression and classifiers |
Fatal (n = 10) |
Convalescent EBOV-negative (n = 16) |
Good quality (n = 16) |
Survived (n = 16) |
Control for EBOV-positive samples |
Healthy controls (n = 6) |
Good quality (n = 6) |
Never infected (n = 6) |
Comparator for convalescent samples |