| Primer | Forward sequence | Reverse sequence |
| β-actin | CGTGGGCCGCCCTAGGCACCA | TTGGCCTTAGGGTTCAGGGGG |
| Runx2 | GCCCAGGCGTATTTCAGA | TGCCTGGCTCTTCTTACTGAG |
| Col1a1 | GAGCGGAGAGTACTGGATCG | GCTTCTTTTCCTTGGGGTTC |
| Opn | ATCTGGGTGCAGGCTGTAA | CCCGGTGAAAGTGACTGATT |
| Dmp-1 | TTGGGATGCGATTCCTCTAC | GGTTTTGACCTTGTGGGAAA |
| Sost | GCAGCTGTACTCGGACACATC | TCCTGAGAACAACCAGACCA |
| Dkk-1 | GACAACTACCAGCCCTACCC | GATCTGTACACCTCCGACGC |
| Rankl | TGAAGACACACTACCTGACTCCTG | CCACAATGTGTTGCAGTTCC |
| Opg | ACCCAGAAACTGGTCATCAGC | CTGCAATACACACACTCATCACT |
| TrAcp5 | CGTCTCTGCACAGATTGCAT | AAGCGCAAACGGTAGTAAGG |
| Ctsk | AGGCATTGACTCTGAAGATGCT | TCCCCACAGGAATCTCTCTG |
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