Table 2.
Brine shrimp cytotoxicity assay of test samples of Rubus ulmifolius
| Conc. (μg/ml) | Number of Brine shrimps | Brine shrimps killed (Mean ± SD, n = 3) | |
|---|---|---|---|
| Ru.Cr | Ru.F | ||
| Negative Control | 10 | 00 | 00 |
| 2.5 | 10 | 3 ± 0.47 | 3.23 ± 0.27 |
| 5 | 10 | 3.33 ± 0.27 | 4.11 ± 0.27 |
| 10 | 10 | 4 ± 0 .47* | 5 ± 0 .47** |
| 12.5 | 10 | 5 ± 0.27** | 7.33 ± 0.54*** |
| 25 | 10 | 6.36 ± 0.27** | 9.33 ± 0.27*** |
| 50 | 10 | 7.42 ± 0.27*** | 9.66 ± 0.27*** |
| 75 | 10 | 9.33 ± 0.27 | All dead |
| 100 | 10 | All dead | All dead |
| 250 | 10 | All dead | All dead |
| 500 | 10 | All dead | All dead |
| LC50 (μg/ml) | 16.7 ± 1.4 | 10.6 ± 1.8 | |
All values are taken as mean ± SD, **P < 0.05, ***P < 0.01