Fig. 3.
Nonphosphorylatable BIM (S55A, S65A, and S100A) enhances IL-3 withdrawal death. Independent clones of FL5.12 BCL-2 cells expressing comparable levels of WT or a serine-substituted BIMEL mutant (BIM S55AS65AS100A) were deprived of IL-3, and cell death was quantitated by Annexin-V-PI staining, 24 h after deprivation. Results are the mean ± SD of three independent experiments. We analyzed four independent clones and the results are reproducible.