Fig. 10.

Effect of in vitro gene silencing on L3 and their in vivo development in jirds. In vitro treatment of L3 with siRNA1 + 2 resulted into reduction in bmugm gene expression and impaired their in vivo development in jirds. a siRNA treatment of L3 resulted in a significant knockdown in bmugm gene expression after 48 h of incubation. b, c L3 incubated with siRNA1 + 2, negative siRNA and in medium for 48 h were inoculated in the peritoneal cavity of jirds followed by euthanization on 120-day post-inoculation for recovery of adult worms which revealed significantly reduced in vivo adult worms establishment in siRNA1 + 2 group. d Significant number of female worms recovered from siRNA-treated group displayed impaired embryogenesis. Percent of sterile females was calculated with respect to total female worms recovered. The fold downregulation in target gene expression in vivo recovered females (e) and males (f) was also quantified by Real time PCR. ***P < 0.001 (very highly significant)