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. 2017 Jan 19;10:34. doi: 10.1186/s13071-017-1967-1

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Knockdown in bmugm gene expression in adult B. malayi female parasites. The relative fold downregulation in mRNA expression of bmugm in B. malayi adult female worms soaked in siRNA1, 2, 1 + 2 was quantified by real time PCR (qRT-PCR) using comparative ΔCT method at different time interval (24, 48, 72, 96, 120 h), β-tubulin was the endogenous control housekeeping gene. The above results depict the mean ± standard deviation (SD) derived from three separate experiments carried out under similar conditions. The gel images depicting the target (bmugm) and endogenous control (β-tubulin) gene expression status at different time points are shown on top (a-e). ***P < 0.001 (very highly significant)