Table 3.
Effect of bmugm gene silencing on viability of adult B. malayi female parasites assessed by motility scoring and MTT reduction assay
| Time (h) | Motility score (female worms)a | % inhibition in MTT reduction (mean ± SD) | |||||||
|---|---|---|---|---|---|---|---|---|---|
| MC | SCR | siRNA 1 | siRNA 2 | siRNA 1 + 2 | SCR | siRNA 1 | siRNA 2 | siRNA 1 + 2 | |
| 24 | 5+ | 5+ | 2+ | 2+ | 2+ | 5.2 ± 0.40 | 66.1 ± 5.58** | 57.9 ± 0.80** | 58.8 ± 9.89** |
| 48 | 5+ | 4+ | 1+ | 1+ | 1+ | 3.7 ± 3.40 | 86.9 ± 1.70*** | 83.4 ± 3.67*** | 87.6 ± 1.9*** |
| 72 | 4+ | 4+ | 1+ | 1+ | 1+ | 11.1 ± 1.84 | 87.3 ± 9.98*** | 88.6 ± 1.58*** | 98.1 ± 2.26*** |
| 96 | 4+ | 4+ | 1+ | 1+ | 1+ | 11.3 ± 3.17 | 88.7 ± 2.47*** | 91.5 ± 1.93*** | 92.2 ± 2.64*** |
| 120 | 4+ | 4+ | 1+ | 1+ | 1+ | 5.3 ± 0.83 | 87.8 ± 3.9*** | 87.4 ± 2.8*** | 90.1 ± 3.73*** |
Abbreviations: MC medium control; MTT (3-(4, 5 dimethylthiazol-2-yl)-2, 5 diphenyltetrazolium bromide); SCR negative control (scrambled siRNA); SD standard deviation
**P < 0.01; ***P < 0.001
a5, 0% motility reduction; 4, 1–25% reduction; 3, 26–49% reduction; 2, 50–74% reduction; 1, 75–99% reduction; D (death), 100% reduction