Table 4.
Effect of bmugm gene silencing on viability of adult B. malayi male parasites was assessed by motility scoring and MTT reduction assay
| Time (h) | Motility score (male worms)a | % inhibition in MTT reduction (mean ± SD) | |||||||
|---|---|---|---|---|---|---|---|---|---|
| MC | SCR | siRNA 1 | siRNA 2 | siRNA 1 + 2 | NC | siRNA 1 | siRNA 2 | siRNA 1 + 2 | |
| 24 | 5+ | 5+ | 2+ | 2+ | 2+ | 11.6 ± 1.98 | 69.1 ± 9.93** | 69.8 ± 2.90** | 69.9 ± 2.13** |
| 48 | 4+ | 4+ | 1+ | 1+ | 1+ | 5.9 ± 2.95 | 60.7 ± 10.47** | 77.2 ± 8.97*** | 84.9 ± 11.95*** |
| 72 | 4+ | 4+ | 1+ | 1+ | 1+ | 7.1 ± 3.68 | 80.5 ± 9.27*** | 85.70 ± 8.50*** | 92.7 ± 0.88*** |
| 96 | 3/4+ | 3/4+ | 1+ | 1+ | 1+ | 6.2 ± 2.35 | 90.8 ± 3.60*** | 87.3 ± 9.60*** | 90.6 ± 9.53*** |
| 120 | 3/4+ | 3/4+ | 1+ | 1+ | 1+ | 7.9 ± 4.13 | 85.2 ± 2.75*** | 83.5 ± 9.85*** | 90.9 ± 1.83*** |
Abbreviations: NC negative control (scrambled siRNA); MC medium control, MTT (3-(4, 5 dimethylthiazol-2-yl)-2, 5 diphenyltetrazolium bromide); SCR negative control (scrambled siRNA); SD standard deviation
**P < 0.01; ***P < 0.001
a5, 0% motility reduction; 4, 1–25% reduction; 3, 26–49% reduction; 2, 50–74% reduction; 1, 75–99% reduction; D (death), 100% reduction