Table 6.
Percentage reduction in motility of microfilariae released by B. malayi female adult parasites after in vitro RNA interference was analyzed by scoring their motility
| Time (h) | Reduction in microfilaria motilitya | ||||
|---|---|---|---|---|---|
| MC | SCR | siRNA1 | siRNA 2 | siRNA1 + 2 | |
| 24 | 5+ | 5+ | 2+ | 2+ | 1+ |
| 48 | 5+ | 5+ | 1+ | 1+ | 1+ |
| 72 | 5+ | 5+ | 1+/D | 1+/D | 1+/D |
| 96 | 5+ | 5+ | 1+/D | 1+/D | 1+/D |
| 120 | 5+ | 5+ | 1+/D | 1+/D | 1+/D |
Abbreviations: MC medium control; SCR negative control (scrambled siRNA)
a5, 0% motility reduction; 4, 1–25% reduction; 3, 26–49% reduction; 2, 50–74% reduction; 1, 75–99% reduction; D (death), 100% reduction