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. 2016 Oct 13;213(3):1168–1180. doi: 10.1111/nph.14223

Figure 1.

Figure 1

Analysis of the transcriptional response under white light of wild‐type (WT; Columbia (Col‐0)) and gun1gun5 Arabidopsis thaliana seedlings treated with either 5 μM norflurazon (NF) or a far‐red light (FR) pretreatment. (a) Heatmap depicting a gene cluster analysis of microarray data for nuclear genes inhibited (blue) or induced (red) at least two‐fold by either NF or FR pretreatment in WT in two independent biological replicates. For the FR gun1gun5 column, expression is shown as the difference between treatment and controls in gun1gun5 relative to the difference in WT. For the NF gun1gun5 column, expression is shown as the difference between NF‐treated WT and gun1gun5. (b) Venn diagram demonstrating the number of genes inhibited (blue) or induced (red) at least two‐fold by NF or an FR pretreatment, and the number of genes rescued 1.5‐fold in gun1gun5 (yellow). (c) Heat map depicting microarray analysis of inhibited (blue) or induced (red) photosynthesis‐related genes grouped by photosynthetic complex. Ratios are the mean of two independent experiments. Columns are represented as in (b). (d) Real‐time reverse transcription−polymerase chain reaction (RT‐PCR) analysis of representative photosynthesis‐related genes. For the NF experiments, WT and gun1gun5 seedlings were grown in the presence (WN and GN) or absence (WC and GC) of 5 μM NF with and without 1.5% (w/v) sucrose. For the FR pretreatment experiments, WT, gun1gun5 or phytochrome A (phyA) mutant seedlings were grown with a pretreatment of FR (WF, GF and AF, respectively) or kept in darkness (WD, GD and AD, respectively). Data shown are the mean ± SE (n = 3 (NF) or n = 7 (FR) independent experiments) with array data represented by green dots, normalized to the WD (FR) or WC (NF) real‐time RT‐PCR values. gun, genomes uncoupled;PSA, photosystem I (PSI) subunit;FD, FERREDOXIN;FNR, ferredoxin:NADP(H) oxidoreductase; PSB,PSII subunit; PET, cytochrome b 6 f subunit;ATPD,ATP synthase subunit;PDE, PIGMENT DEFECTIVE;LHC, LIGHT HARVESTING CHLOROPHYLL A/B BINDING PROTEIN; ELIP, EARLY LIGHT‐INDUCIBLE PROTEIN.