Figure 1.

EU individuals show elevated IgG reactivity to HCV envelope proteins which correlates with lifetime risk of exposure. (a+b) Purified IgGs were tested for their ability to bind HEK‐293T‐expressed E1E2 in a (a) GNA capture gt 1a and (b) GNA capture gt 3a ELISAs. For those individuals where serum from multiple time points was available, each time point was tested individually, but only one value for each individual (i.e. the first sample taken) is plotted. Statistical differences between the groups were calculated using the Wilcoxon rank sum test (*P<.05). (c+d) Correlation between IgG reactivity to either gt 1a (c) or gt 3a (d) and total number of lifetime injecting episodes (duration of reported injection drug use multiplied by the frequency of reported injection drug use) was plotted for 37 EU (injection data not available for the remaining 5)