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IgG and IgM responses to soluble gt 1a E2 are evident in EU individuals. IgG (a) and IgM (b) absorbance to purified gt1a sE2 protein was determined for EU and HC individuals using a modified ELISA protocol described in the Supplementary Methods. Statistical differences between the groups were calculated using the Wilcoxon rank sum test. **P<.01, *** P<.001
