Figure 3.
Neutralization of HCVpp by purified IgGs from EU. Virus pseudoparticle neutralization assays were performed using HCVpp bearing E1E2 derived from (a and c) gt 1a strain H77 or (b) gt 3 HCV. (a and b) Percentage reduction in HCVpp entry after incubation with 400 μg mL −1 test IgG is plotted as evidenced by luciferase reading at 72 hours compared to control. (c) Percentage reduction in HCVpp entry after incubation with reducing concentrations of IgG as shown. The 50% cut‐off is shown as a dashed lined. All results represent the average of at least 3 separate technical replicates, with error bars representing the SEM