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. 2017 Jan 19;18:12. doi: 10.1186/s13059-016-1140-8

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Viral detection and viral biomarker qPCR results with RNA-seq subject selection. Expression of CCL8 and CXCL11 in 161 subjects was screened by qPCR. Respiratory viruses were detected by qPCR in the samples colored red.; all of those subjects were then sequenced with RNA-seq. Samples colored in orange show the two subjects with suspected viral infection, despite viral qPCR negative results, based on the expression of CCL8 and CXCL11; both of those subjects were selected for RNA sequencing. Samples colored in blue represent subjects with no respiratory viruses identified with qPCR and selected for the RNA-seq part of the study. The remaining samples (in gray) represent subjects with no respiratory viruses identified with qPCR and not selected for the RNA-seq part of the study. Shape of the points represents asthma status, while the size corresponds to 2^(−Ct) value for virus detection by qPCR