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. 2017 Jan 19;8:7. doi: 10.1186/s40104-017-0141-0

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Archaea:bacteria relative abundance in relation to methane emissions, preliminary data from the 1000-cow RuminOmics project. Dairy cows on different farms throughout Europe received grass or maize silage:concentrate diets of similar nutrient composition. Feed intake was measured either directly or calculated from faecal long-chain hydrocarbons. Samples of rumen contents were removed by stomach tube and DNA was extracted by the Yu & Morrison method [110]. Abundances were calculated from qPCR of 16S rRNA genes using universal primers for archaea and bacteria