Fig. 3.

Neighbor Joining tree of Prevotella-like OTUs that had a negative (blue dots) or positive (red dots) relation to methane (expressed in terms of g methane/kg DMI) in the 1,000-cow RuminOmics project. Multiple alignment was done using MUSCLE [111]. The Neighbor Joining tree was constructed using p-distance and pairwise-deletion parameters. The tree was resampled 1,000 times and bootstrap values are indicated. The linearized tree was computed using MEGA v5.1 [112] by using most abundant Bacteroidales OTUs to create an “outgroup”