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. 2017 Jan 5;2017:1654237. doi: 10.1155/2017/1654237

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Copyright © 2017 Peer H. A. Timmers et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Phylogenetic tree of full length archaeal 16S rRNA sequences showing all methanotrophic clades so far described (grey) and other archaeal clades used in our domain based (meta)genome comparison (black). The tree was constructed with the ARB software package (version arb-6.0.1.rev12565) [49] using 2800 sequences from the SILVA SSURef NR 99 database (release 119.1) [50]. Trees were calculated by maximum likelihood analysis (RAxML, PHYML) and the ARB neighbor-joining method with terminal filtering and the Jukes-Cantor correction. Resulting trees were compared manually and a consensus tree was constructed. Sulfolobales as outgroup was removed after tree calculations. The scale bar represents the percentage of changes per nucleotide position.