Table 2.

Cre driver lines used for Mc4r manipulation

Cre driver line	Type	Selectivity	Main sites of neuroanatomical overlap with Mc4r	Ref.
Nestin (Nes)	Transgene	Pan-neuronal	All sites	53
vGlut2 (Slc17a6)	Knock-in	Vesicular glutamate transporter (excitatory neurons)	Most glutamatergic sites	54
vGat (Slc32a1)	Knock-in	Vesicular GABA transporter (inhibitory neurons)	Most GABAergic sites	54
Sim1	Transgene	Single-minded 1	PVH, MeA, NLOT, PH	53
Chat	Knock-in	Choline acetyltransferase (cholinergic neurons)	DMV, IML, LDTg, PPTg	69
Phox2b	Transgene	Paired-like homeobox 2b	DMV, NTS, NG	69
Drd1	Transgene	Dopamine-1 receptor	NAc, PVH,	49
Oxt	Knock-in	Oxytocin	PVH	54
Avp	Knock-in	Arginine vasopressin	PVH	54
Pdyn	Knock-in	Pro-dynorphin	PVH, VMH, DMH, LH, DRN, LPBN	54
Crh	Knock-in	Corticotropin-releasing hormone	PVH	54

Cre driver lines used for manipulation of Mc4r conditional alleles. The genetic nature of each Cre line and its neuronal selectivity is provided. The listing of main sites of anatomical overlap defines only the most abundant sites of neuroanatomical coexistence between Mc4r and each Cre driver gene and not necessarily cellular coexpression. DMH, dorsomedial nucleus of the hypothalamus; DMV, dorsal motor nucleus of the vagus; DRN, dorsal raphe nucleus; IML, intermediolateral nucleus of the spinal cord; LDTg, laterodorsal tegmental nucleus; LH, lateral hypothalamus, LPBN, lateral parabrachial nucleus; MeA, medial amygdala; NAc, nucleus accumbens; NG, nodose ganglion; NLOT, nucleus of the lateral olfactory tract; NTS, nucleus of the solitary tract; PH, posterior hypothalamus; PPTg, pedunculopontine tegmental nucleus; PVH, paraventricular nucleus of the hypothalamus; VMH, ventromedial nucleus of the hypothalamus.