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Effect of nicotine on EPC number. EPCs positive for (A) acetylated low density lipoprotein; (B) fluorescein isothiocyanate-conjugated Ulex europaeus agglutinin, and (C) double-positive cells. (D) Statistical analysis of EPC number. *P<0.05 vs. C-1 M group; **P<0.05 vs. C-3 M group; ***P<0.05 vs. C-6 M group. Cells were extracted from 3 mice. EPCs, endothelial progenitor cells; C, control; M, month(s); N, nicotine-treated.
