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p16 immunostaining in CIN. (A) Complete lack of immunostaining or positivity confined to the lower third of the squamous epithelium (−/+) in CIN1 (magnification, ×10). (B) Positivity confined up to two thirds of squamous epithelium (++) in CIN2 (magnification, ×10). (C) Positivity is diffused in all epithelial levels (+++) in CIN3 (magnification, ×20). CIN, cervical intraepithelial neoplasia.
