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. 2017 Jan 17;5:e2866. doi: 10.7717/peerj.2866

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©2017 Lemes et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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Ligand blot analysis of the binding of Cry1Aa (1), Cry1Ac (2) and Vip3Aa (3) toxin to BBMV proteins from D. flavipennella (A) and E. lignosellus (B). BBMVs proteins (40 µg) from each insect were transferred to PVDF membrane and incubated with 40 nmol of biotinylated toxins. Arrows indicate main BBMV proteins recognized by each toxin in each BBMV sample.