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Effect of psammaplin A on SV40 DNA replication in vitro. Replication reaction comprised SV40 origin-containing DNA (pUC-ori+), SV40 T-Ag, HeLa cytosolic extract (100 μg), [3H]dTTP, and the indicated amounts of psammaplin A. Reaction mixtures were incubated at 37°C for 2 hr, after which the acid-insoluble radioactivity were measured. Data are shown as means ± SD of three independent experiments. Data are significantly different from control group at p < 0.05 (*)and at p < 0.001 (**).
