Fig. 3.

Western blotting and RNA sequencing from HCMV infected cells treated with XMD7-1. (A) Organization of HCMV sequences encoding IE1/2 proteins and IE1/2 proteins produced during HCMV replication. The 5 exons of the HCMV UL122-123 locus that encode IE1 and IE2 proteins are shown. Black arrows in exons 2 and 5 represent start codons. Above the exons are the exon-exon junctions. Below the exons the alternative spicing of RNAs that encode IE1 and IE2 proteins is shown, as are IE2 proteins IE2-60 and IE2-40 produced from internal start sites in exon 5. (B and D) HFF cells were infected with AD169 at an MOI of 1, then treated with either 1 μM XMD7-1 or the equivalent volume of DMSO at the time of infection. Cell lysates were prepared for western blotting at the time points (hours post infection (h.p.i.)) indicated above the figure. Uninfected cells harvested at the time of infection are shown as 0 h.p.i. Proteins recognized by the antibodies used are indicated to the right of each figure. The positions of molecular mass markers (kDa) are indicated to the left of each figure. (C) Number of HCMV RNA sequence reads from total RNA (excluding rRNA) from HFF cells infected with AD169 at an MOI of 1, then treated with either 1 μM XMD7-1 or the equivalent volume of DMSO at the time of infection.