Figure 4.

H19 suppression changed expression level of epithelial-mesenchymal transition molecules in HEC-1-B cells. (A) The expression level of E-cadherin and vimentin in cells exposed to siNC, siH19-a or siH19-b was monitored by quantitative polymerase chain reaction and western blot analysis, with β-actin as an internal control. Knockdown of H19 increased E-cadherin at the mRNA level and protein level, but caused no evident change in vimentin expression. (B) Subsequent to H19 small interfering RNA transfection, mRNA and protein levels of Snail were examined, and standardized against the level present in negative control cells using β-actin as a loading control. H19 suppression significantly decreased Snail expression level in HEC-1-B cells. All treatments in this fig. were performed in triplicate, and values are presented as the mean ± standard deviation of the three experiments. P<0.05 was considered significantly different for t-test. siNC, small interfering negative control, siH19, small interfering RNA specific to H19.