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. 2016 Nov 22;13(1):45–50. doi: 10.3892/ol.2016.5410

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Copyright: © Tajima et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — (A) TLC of the constituents of the Gb₃Cer and Gb₄Cer bands isolated from well- (bands T1 and G1) and poorly (bands T2 and G2) differentiated endometrial adenocarcinoma. Neutral glycolipids were isolated from the total neutral lipid fraction as described in Materials and methods. The total neutral glycolipids and the isolated glycolipids were developed with chloroform/methanol/water (65:35:8, v/v) and visualized with orcinol-H₂S0₄ reagent. Lanes Well and Poorly correspond to the total neutral glycolipids in well- and poorly differentiated adenocarcinoma, respectively. Lanes T1, T2, G1 and G2 are the isolated neutral glycolipids from the three bands of Gb₃Cer and the band of Gb₄Cer, respectively. (B) TLC schema of T1, T2, G1 and G2. TLC, thin-layer chromatography; GlcCer, glucosylceramide; LacCer, lactosylceramide; Gb₃Cer, globotriaosylceramide; Gb₄Cer, globotetraosylceramide; Lc₄Cer, lactotetraosyl ceramide.